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. Author manuscript; available in PMC: 2007 Apr 10.
Published in final edited form as: Mitochondrion. 2005 Aug;5(4):235–247. doi: 10.1016/j.mito.2005.01.004

Fig. 1.

Fig. 1

Western blot analyses of ERK1/2 phosphorylation in HLE-B3 and BLECs with E2 or serum. Total cell lysates (20 μg protein/lane) from quiescent HLE-B3 (A) and BLEC (B) cultures serum-starved for at least 18 h prior to stimulation by either 1 μM E2 or 1% serum for 0, 5, 15, 30, and 60 min. Membranes were stripped and reprobed with an antibody to actin as a control for protein loading. This experiment was repeated three times with similar results.