Skip to main content
. 2001 Jan;158(1):299–303. doi: 10.1016/s0002-9440(10)63969-7

Figure 4.

Figure 4.

Treatment with 5-Aza-CdR induces demethylation and RAR β2 protein expression in breast cancer cell lines. T-47D and MRK-nu-1 cells were left untreated or incubated for 3 days with 5-Aza-CdR. A: Methylation-specific PCR analysis of cell line DNA before (lanes 1, 2, 5, and 6) and after (lanes 3, 4, 7, and 8) 5.0 μmol/L 5-Aza-CdR treatment. Extracted DNA was treated with bisulfite as described in the text, and amplified by PCR using primers specific for methylated (M lanes) and unmethylated (U lanes) DNA. The presence of a 146-bp PCR product in lanes marked U indicates that the corresponding RAR β2 gene is unmethylated; product in lanes marked M indicates that RAR β2 is methylated. B: Western blot analysis of RAR β2 protein expression in cell lines before and after treatment for 3 days with 1.0 μmol/L (lanes 2 and 5) and 5.0 μmol/L (lanes 3 and 6) 5-Aza-CdR, in comparison with untreated cells (lanes 1 and 4).