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. 2001 Jan;158(1):227–233. doi: 10.1016/S0002-9440(10)63961-2

Figure 5.

Figure 5.

AviII restriction site analysis of neuroserpin exon 2 PCR products. The A to C transition at the first position corresponding to codon 52 of neuroserpin creates an AviII restriction site within the 167-bp PCR-amplified fragment when coupled with mutagenesis primer (NSz). Enzyme digestion gives fragments of 142 bp and 25 bp (the latter is not visible) for the mutant allele. The PCR-amplified fragments (167 bp) of the proband (lane 1) and his affected brother (lane 2) were incompletely digested, suggesting that they are heterozygous for this mutation. The fragment from a normal control is not digested with AviII (lane C). M: 100-bp molecular ruler DNA size standard (Bio-Rad).