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. 2001 Feb;158(2):627–637. doi: 10.1016/S0002-9440(10)64004-7

Figure 6.

Figure 6.

Isolation of immune complexes from baboon blood after pulmonary, renal, or cardiac xenotransplantation. Serum isolated from baboon blood after pulmonary, renal, or cardiac xenotransplantation was diluted 5:1 with 12% PEG 8,000 and 60 mmol/L EDTA in VBS for 16 hours at 4°C to precipitate immune complexes. The solution was centrifuged at 2,000 × g for 20 minutes at 4°C to pellet immune complexes. Precipitates were resuspended in PBS, reduced, separated by SDS-PAGE (7.5% PA) and transferred to PVDF and stained as follows. Lane 1, pretransplant baboon serum reacted with human serum and then with anti-human IgM. Lane 2, baboon serum, taken 1 hour after xenotransplantation of a porcine lung, reacted with human serum and then with anti-human IgM. Lane 3, baboon serum, taken 2 hours after xenotransplantation of a porcine lung, reacted with human serum and then with anti-human IgM. Lane 4, baboon serum, taken 4 hours after xenotransplantation of a porcine lung, reacted with human serum and then with anti-human IgM. Lane 5, pretransplant baboon serum reacted with human serum and then with anti-human IgM. Lane 6, baboon serum taken 30 minutes after xenotransplantation of a porcine kidney, reacted with human serum and then with anti-human IgM. Lane 7, baboon serum taken 60 minutes after xenotransplantation of a porcine kidney, reacted with human serum and then with anti-human IgM. Lane 8, pretransplant baboon serum reacted with human serum and then with anti-human IgM. Lane 9, baboon serum taken 15 minutes after xenotransplantation of a porcine heart, reacted with human serum and then with anti-human IgM. Lane 10, baboon serum taken 30 minutes after xenotransplantation of a porcine heart, reacted with human serum and then with anti-human IgM. Lane 11, baboon serum taken 60 minutes after xenotransplantation of a porcine heart, reacted with human serum and then with anti-human IgM. A protein in the blood of baboons after pulmonary, renal, and cardiac xenotransplantation with an apparent molecular weight of 270 kd is reactive with human xenoreactive IgM.