Figure 2.

Dual immunofluorescent staining of IL-1RII (A) and IL-1β (B) in the endometrial tissue of normal women. Tissue sections were successively incubated with mouse monoclonal anti-IL-1RII antibody, rabbit polyclonal anti-IL-1 antibody, and biotinylated goat anti-rabbit antibody before being incubated simultaneously with rhodamine-conjugated goat anti-mouse antibody and fluorescein isothiocyanate-conjugated streptavidin. Serial sections incubated with normal mouse and normal rabbit IgGs instead of the primary antibodies were included as negative controls (C and D) (original magnification, ×160). Note the co-expression of IL-1RII (red color) and IL-1β (green-yellow color) within the luminal deposit in endometrial glands. Data from a normal woman in the secretory phase of the menstrual cycle (day 23).