Figure 4.

Western blot analysis of IL-1RII expression in the endometrial tissue. A: Normal women: day 10 (lanes 1 and 3) and day 24 (lanes 2 and 4). B: Women with endometriosis: stage II, day 13 (lanes 1 and 3) and stage II, day 25 (lanes 2 and 4). Equal amounts of endometrial proteins (100 μg/lane) were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to nitrocellulose membranes. IL-1RII was detected using a mouse monoclonal antibody (lanes 1 and 2) and the immunocomplex was revealed by chemiluminescence. No immunoreaction was observed in negative controls where the anti-IL-1RII antibody was replaced by an equal concentration of mouse immunoglobulins of the same isotype (lanes 3 and 4).