Figure 5.

Protein overlay assay. A: Influence of the 1287ins3 mutation on the binding of integrin β4 to plectin. Increasing concentrations (10 to 1,000 nmol/L) of Eu³⁺-labeled integrin β4 were incubated with coated (100 nmol/L) wild-type (closed circles) and mutated (open circles) recombinant proteins, as well as BSA (closed triangles). All data are presented as the mean ± SD of triplicate determinations. B: Increased self-binding of recombinant plectin proteins containing the 1287ins3 mutation. Wild-type or mutated recombinant proteins, as well as BSA, were coated onto microtiter plates (100 nmol/L) and overlaid with increasing concentrations (10 to 500 nmol/L) of Eu³⁺-labeled wild-type (circles) or mutated (triangles) recombinant proteins.