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. 2001 Feb;158(2):723–734. doi: 10.1016/S0002-9440(10)64015-1

Figure 7.

Figure 7.

RT-PCR analysis of TIMP gene expression in normal and diabetic corneal epithelium (left) and stroma (right). Left: Normal corneas (lanes 1 to 5); non-DR diabetic corneas (lanes 6 to 13); and DR corneas (lanes 14 to 18). Right: Normal corneas (lanes 1 to 3); non-DR diabetic corneas (lanes 4 to 7); and DR corneas (lanes 8 to 10). −RT, negative control without RT. No change in epithelial expression of all genes is seen. A slight overexpression of TIMP-2 and TIMP-3 genes in diabetic corneal stroma was not statistically significant. Autoradiograms of Southern blots are shown. Samples were normalized with respect to β2-MG cDNA amplification.