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. 2001 Feb;158(2):723–734. doi: 10.1016/S0002-9440(10)64015-1

Table 1.

RT-PCR Primers and Hybridization Oligonucleotides Used in this Study

Gene Forward primer Reverse primer Product size, bp Hybridization oligonucleotide
α1 LN AAGTGTGAAGAATGTGAGGATGGG CACTGAGGACCAAAGACATTTTCCT 441 CCTGCGTACAGTGTTCCTTGGGTTACAGAG
α5 LN CTGAGGACTGAAGTGAAAACTCAAG AACTGGTAGGAGTCTCGGGTG 473 GTCTGGCACCATTTACAACTTCAGTGG
β1 LN CCAAGATCCTTATCATGAGACCCTG GATCTAAAGCACGAAATATCACCTC 342 GTATAGATACTTCGCCTATGACTGTGAG
N-1/EN CTGGGGAAGGTTTATTATCGAG TGAGAATGTCGTATGGAACTGC 276 GGAGATCTCTTTCCAGCCTAGTAGCGCGGTGGT
α3 INT TACTACTTCGAGAGGGAAAGAGGAAG GAAGGTCTGGGTAGAAGTTCTCATC 358 GCAAAGTGTACATCTATCACAGTAGCTCTA
β1 INT TTATCCTTCTATTGCTCACCTTGTC ATAACCTCTACTTCCTCCGTAAAGC 426 CCTAAGTCAGCAGTAGGAACATTATCTG
MMP-1* CGACTCTAGAAACACAAGAGCAAGA AAGGTTAGCTTACTGTCACACGCTT 786 ND
MMP-2 GGGACAAGAACCAGATCACATAC CTTCTCAAAGTTGTAGGTGGTGG 446 AATGGCAAGGAGTACAACAGCTGCACTGAT
MMP-3 GAACAATGGACAAAGGATACAAC AAATGAAAACGAGGTCCTTGCTAG 460 CGGAACCTGTCCCTCCAGAACCTGGGAC
MMP-7* GGTCACCTACAGGATCGTATCATAT CATCACTGCATTAGGATCAGAGGAA 373 ND
MMP-9* GTATTTGTTCAAGGATGGGAAGTAC GCAGGATGTCATAGGTCACGTAG 515 ND
MMP-10 ACCTGGGCTTTATGGAGATATTC TCATATGCAGCATCCAAATATGATG 452 CAAAATCTGTTCCTTCGGGATCTGAGATG
MMP-14 CGCTACGCCATCCAGGGTCTCAAA CGGTCATCATCGGGCAGCACAAAA 497 CATAATGAAATCACTTTCTGCATCCAGAATTACACC
TIMP-1 CTGTTGTTGCTGTGGCTGATAG CTTTTCAGAGCCTTGGAGGAC 507 ACCTTATACCAGCGTTATGAGATCAAGATGACC
TIMP-2 TCTGGAAACGACATTTATGG GTTGGAGGCCTGCTTATGGG 501 TGGGGTCTCGCTGGACGTTGGAGGAAAGAAGGAA
TIMP-3 CATCAAGCAGATGAAGATGTACC GGTAGTAGCAGGACTTGATCTTGC 269 CTCACCCTCTCCCAGCGCAAGGGGCTGAACTATC
CV/L2† TACGGCTTTGAAGGAGCAAAT AGAATTAAGCAATGAGTCCTTTGA 378 GGACAGCATGTCTGGGGAAATTTTATCTTGAAACTG
U-PA CACACACTGCTTCATTGATTACC TGGTGACTTCAGAGCCGTAGTAG 404 TCGTCTACCTGGGTCGCTCAAGGCTTAACTCCAACA
T-PA TGTGTGGAGCAGTCTTCGTTTC CCTGGTATCTATTTCACAGCAC 334 GAGCCAGATCTTACCAAGTGATCTGCAGAGATGAA
β2-MG CTCGCGCTACTCTCTCTTTCTG GCTTACATGTCTCGATCCCACTT 335 GTCTTTCAGCAAGGACTGGTCTTTCTATCTCTTGTA

LN, laminin; N-1/EN, nidogen-1/entactin; INT, integrin; CV/L2, cathepsin V/L2; U-PA, urokinase-type plasminogen activator; T-PA, tissue plasminogen activator; β2-MG, β2-microglobulin; bp, base pairs; ND, not designed. *, from Giambernardi et al.; †, from Adachi et al. All primers are from 5′ to 3′.