Table 2.
Antibodies to Proteinases Used for Immunostaining in this Study
| Proteinase | Antibody and name | Use for immunostaining | Source |
|---|---|---|---|
| MMP-1/collagenase | mAb VI3 | Good | NeoMarkers |
| mAb COMY 4A2 | Weak staining | NeoMarkers | |
| mAb 41-1E5 | Unusual staining | Chemicon | |
| MMP-2/gelatinase A | mAb 42-5D11 | Nonspecific staining | Chemicon |
| mAb CA-4001* | Nonspecific staining | NeoMarkers | |
| mAb VB3 | Weak staining | NeoMarkers | |
| mAb VID2 | Good | NeoMarkers | |
| mAb A-Gel VC2* | Unusual staining | NeoMarkers | |
| mAb Gel-B IIA5 | Weak staining | NeoMarkers | |
| mAb KO VIIB1 | Some nonspecific staining | NeoMarkers | |
| Sheep pAb 0211 | Weak staining | Biogenesis | |
| MMP-3/stromelysin-1 | mAb SL-1 IIIC4 | Weak staining | Chemicon |
| mAb 55-2A4 | Good | Calbiochem | |
| mAb 10D6 | Good | R&D Systems | |
| mAb SL-1 IID4 | No staining detected | L.J. Windsor | |
| Rabbit pAb AB812 | Also detects MMP-10 | Chemicon | |
| MMP-7/matrilysin | mAb ID2 | Good | NeoMarkers |
| MMP-9/gelatinase B | mAb GE-213 | Good, stronger than pAb | NeoMarkers |
| mAb IA4 | Weak staining | NeoMarkers | |
| mAb IA5 | No staining detected | NeoMarkers | |
| mAb IIA5 | Weak staining | NeoMarkers | |
| mAb IVA2 | Some nonspecific staining | NeoMarkers | |
| mAb VIIC2 | Weak staining | NeoMarkers | |
| mAb 56-2A4 | Weak staining | Chemicon | |
| Sheep pAb 0911 | Good | Biogenesis | |
| MMP-10/stromelysin-2 | mAb IVC5 | Good, somewhat weak | L.J. Windsor† |
| mAb IB1 | No staining detected | NeoMarkers | |
| mAb VC3 | Weak staining | NeoMarkers | |
| mAb IIA1 | No staining detected | NeoMarkers | |
| mAb IIIB3 | Mostly stains epithelium | L.J. Windsor | |
| mAb IID6 | No staining detected | L.J. Windsor | |
| mAb IIID1 | Weak staining | L.J. Windsor | |
| mAb 142-9B11 | Unusual staining | Chemicon | |
| Sheep pAb D248-6 | Good | G. Murphy | |
| MMP-14/MT1-MMP | mAb 114-6G6 | Good | Chemicon |
| Urokinase | Goat pAb #398 | Weak staining | American Diagnostica |
| mAb #394 | Some nonspecific staining | American Diagnostica | |
| mAb 2B2.10.6 | Good | Serotec | |
| Rabbit pAb 8701 | Good | L.J. Windsor | |
| Rabbit pAb 8703 | Good, stronger than 8701 | L.J. Windsor | |
| Goat pAb AB776 | High background | Chemicon | |
| Tissue plasminogen activator | mAb PAM-3 | Good | American Diagnostica |
| Procathepsin V/L2 | Rabbit pAb AS-923 | Good | W. Adachi/S. Kinoshita |
Unusual staining, the pattern did not match those of any other antibody to the same MMP.
*The antibody recognizes only the proform of MMP-2.
†Also available from NeoMarkers.
mAb, mouse monoclonal antibody; pAb, polyclonal antibody.