Figure 3.

Autoradiographic detection of the MMP-1 SNP and quantification of LOH in tumor tissue. Data from the 72-bp amplicon assay (A) and the 82-bp amplicon assay (B) are shown. Normal tissue/tumor pairs from three patients are at the left; standards, composed of known mixtures of DNA from 1G and 2G homozygous controls, are at the right. The “%2G” shown for the tumor samples was determined by quantitative phosphorimager analysis and calculated from a standard curve (see Figure 4 ▶ ) constructed from standards run on the same gel. Tumors from patients 65 and 34 have lost the 1G allele. The tumor from patient 22 has lost the 2G allele.