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RT-PCR analysis of cytokine mRNA expression. Total RNA was isolated from lung tissues of NF-IL-6 KO and WT mice infected with M. tuberculosis and subjected to RT-PCR using cytokine-specific primers. G-CSF mRNA expression is lower in the lung of NF-IL-6 mice than that of WT mice. Lung fibroblasts from NF-IL-6 KO mice G-CSF-mRNA significantly.
