Figure 7.

Up-regulation of CA9 and CA12 mRNA expression correlates with media acidification. 786-0 cells were harvested at different stages of the growth curve and levels of CA9 and CA12 transcripts were assessed by Northern hybridization. In parallel, glucose consumption (not shown) and changes in media pH were monitored. The onset of confluent stage (day 2) is indicated by an arrow. Enhanced acidification of the media by the VHL-deficient cell line 786-0 is pronounced at the late confluent stage (day 7, boxed) and is paralleled by up-regulation in CA9 and CA12 mRNA expression.