Figure 2.

Top: Western blot analysis of caveolin-1 expression in normal mesenchymal tissues and sarcomas. In normal mesenchymal tissues (lanes 1 and 2) and three tumors (lanes 3, 5, and 7) with a high expression of caveolin-1 in immunohistochemistry (Table 2 ▶ ; cases 1, 3, and 5) the 24-kd [α]- and the 21-kd [β]-isoforms of caveolin-1 were detectable. In two tumors (lanes 4 and 6) with a reduced expression of caveolin-1 in immunohistochemistry (Table 2 ▶ , cases 2 and 4) no caveolin-1 protein was detected. Bottom: To ensure equal loading amounts, the blots were stripped and reprobed with an antibody to actin.