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Expression of nestin in GISTs determined by immunohistochemistry. A–E: Sections of case 1 (GIST) are shown. A: Staining with H&E. B: Immunostaining with anti-KIT. C: Immunostaining with anti-CD34. D: Immunostaining with anti-α-SMA. E: Immunostaining with anti-nestin. Marked immunoreactivity for nestin is observed in GIST. Asterisks in A to E indicate nontumor mature smooth muscle cells that are positive for α-SMA. Original magnification, ×73.
