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. 2001 Mar;158(3):1029–1038. doi: 10.1016/S0002-9440(10)64050-3

Figure 3.

Figure 3.

Unfixed frozen sections of a spleen with AA amyloid deposits were incubated with cathepsin K at pH 5.5 in the presence (A and C) and absence (B and D) of E64. In the presence of E64 amyloid deposits showed immunostaining for AA amyloid at the margins only (A). In the absence of E64 immunostaining was also present in the center of the deposits (B). Immunostaining for amyloid P component was not affected by cathepsin K pretreatment and it was predominantly confined to the margins of the deposits (C and D). Immunostaining with anti-AA amyloid (A and B) and anti-amyloid P component (C and D); hematoxylin counterstain. Original magnification, ×100.