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. 2001 Jul;159(1):369–379. doi: 10.1016/S0002-9440(10)61703-8

Figure 7.

Figure 7.

CDK activities and CDK/p27 complex formation in K5CDK4 mouse epidermis. A: Kinase activity of CDK4 and CDK2 from K5CDK4 (T) and normal sibling animals (wt). Fresh epidermal proteins were immunoprecipitated with specific antibodies and in vitro kinase assays were performed with a pRb peptide as substrate. B: Fresh epidermal proteins from transgenic (T) and wild-type mice (wt) were immunoprecipitated with a polyclonal antibody against p27Kip1 and immunoblotted with polyclonal antibody for CDK4 or p27Kip1. The control was immunoprecipitated with normal rabbit serum (NR).