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Protein carbonylation determined by two-dimensional Western blot immunoassay. Crude extracts from cells treated with 0 and 1 μg/ml of streptomycin and 200 μM hydrogen peroxide were processed for resolution on two-dimensional polyacrylamide (19) with modifications (20). Autoradiograms were obtained after carbonyl immunoassay of proteins (5). The analysis was repeated three times to confirm reproducibility. Representative results are shown.
