Figure 3.

Level of expression of the Smad pathway elements in fibroblasts and myofibroblasts. Total protein extracts from fibroblasts and myofibroblasts cultivated to 70 to 80% confluence were used to compare by Western blotting the amount of the indicated Smad pathway elements. Glyceraldehyde-3-phosphate dehydrogenase was used as a control of protein loading. The positions of the molecular weight markers are indicated on the right. For each protein analyzed, the figure represents a mount of a single film.