Table 3.
Determination of VH, D and JH Usage and Nucleotide Sequence of CDR3 Region in Nodal MZL
| Case no. | VH | N | D | N | JH |
|---|---|---|---|---|---|
| 1 | TGT GCGAGA | ggcccc | GATAGcAGTGGaTATTACTAC | tt | CTAC TGG |
| VH1-69 | D3-22 | JH4 | |||
| 2 | TGT GCGAGAGGT | agaaagggag | GCAGTGGCTtGTAC | ctgaaacat | ATGgTTTgGAcGTC TGG |
| VH4-34 | D6-19 | JH3 | |||
| 3 | TGT GCGAGAGGT | ggctatgatagtgatgg | CTccgTGGGGA | cgatggtc | GCTTTTGATATC TGG |
| VH4-34 | D7-27 | JH3 | |||
| 4 | TGT GCGAG | ggggccc | GATAGTAGTGcaTATTACTAC | ttt | TAC TGG |
| VH1-69 | D3-22 | JH4 | |||
| 5 | TGT GCGAGA | gggccc | GATAGTAGTGcaTATTACTA | ttt | CTAC TGG |
| VH1-69 | D3-22 | JH4 | |||
| 6 | TGT GCG | —— | actattcgttatcggcgccgcctatctttctttcggtat | —— | TACacCt TGG |
| VH4-34 | JH6 | ||||
| 7 | TGT GCGAAA | gaagggac | TTTTTGGAGTGG | ccc | CTcTGACTgg TGG |
| VH3-30.5 | D7-27 | JH4 | |||
| 8 | TGT GCACAT | tt | GTATTAtTATGATAGTgGTGtTTATTAC | cgcc | ACTTTGACTAC TGG |
| VH2-05 | D3-22 | JH4 | |||
| 9 | TGT GCCAGAGAT | —— | attcctttggtcgggagtataggtcg | —— | TACTGCGGTATGGACGTC TGG |
| VH4-30.4 | JH | ||||
| 10 | TGT GCGAGAGAT | cgattgacggtcg | ACGATTTTTGGAGTG | cca | ACTACTTaGACTAt TGG |
| VH3-48 | D3-3 | JH4 |
Nucleotides of N segments and mutations with respect to the germline sequence are indicated in lowercase.
In cases 6 and 9 a germline D segment was not assigned.