Figure 4.

Effects of IL-4 blockade on antibody responses. All mice received CM immunization on days 0 and 7. Anti-IL-4 and the isotype control mAbs were given as described in Figure 3 ▶ . Sera were collected on days −1, 9, 16, and 21. A: Total IgE levels on days −1 (1 day before the first CM injection), 9, and 21 as measured by a sandwich ELISA (described in Materials and Methods). B: CM-specific IgG1 response on days 9 and 21. C: CM-specific IgG2a response on days 9 and 21. Both CM-specific IgG1 and IgG2a responses were measured using ELISA. All data points represent values from individual mice. Data are representative of three experiments. P values were calculated using the Student’s t-test.