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. 2001 Jul;159(1):273–283. doi: 10.1016/s0002-9440(10)61693-8

Figure 5.

Figure 5.

Effects of poly IC and IFN-γ on DNA integrity of islet cells isolated from PKR−/− and PKR+/+ mice. Islets (100 per 400 μl of complete CMRL-1066) isolated from PKR−/− and PKR+/+ mice were treated for 48 hours with 400 μg/ml of poly IC, 150 U/ml mouse IFN-γ and 1 mmol/L NMMA as indicated. The islets were then isolated, dispersed into individual cells, and DNA damage was determined by TUNEL staining (a). For nitrite production, islets (100 per 400 μl of complete CMRL-1066) isolated from PKR−/− and PKR+/+ mice were treated for 48 hours with 400 μg/ml of poly IC and 150 U/ml of mouse IFN-γ as indicated. The supernatant was removed and nitrite production determined (b). Results are the average ± SEM of three (a) and two (b) independent experiments.