Figure 1.

Competitive ELISA. This graphic representation illustrates the concentration-dependent competition of the immunizing peptide (ERβC) with the GC-17 antibody. In addition, preincubation with 4 mg/ml of the control N-terminus peptide (ERβN) and GC-17 failed to compete out the binding to the recombinant ER-β peptide, demonstrating the specificity of the antibody. The GC-17 antibodies, bound to recombinant ER-β protein on ELISA plates, were recognized by alkaline phosphatase-conjugated anti-rabbit IgG antibodies. The whole complexes were visualized by incubation with p-nitrophenyl phosphate. The optical density at 405 nmol/L was measured. The entire assay was done four times. The column represents the mean value of optical density at 405 nmol/L of four measurements and the bar represents the SD. w/o, GC-17 preincubated without peptide antigen.