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PCR Sequences and Annealing Temperatures
| Locus | Sequence (5′-3′) | Fragment size (bp) | Type of repeat | Annealing temperature |
|---|---|---|---|---|
| D5S592 (F) | AGACAGACAGAGAGATTAGA | 145–205 | Tetranucleotide | 55°C |
| D5S592 (R) | AGTAAAGTGAGTGGAGAGC | |||
| D16S2624 (F) | TGAGGCAATTTGTTACAGAGC | 143 | Tetranucleotide | 55°C |
| D16S2624 (R) | TAATGTACCTGGTACCAAAAACA | |||
| D17S1987 (F) | AAGAGCTGGGGGAGCTTAAG | 185–209 | Tetranucleotide | 57°C |
| D17S1987 (R) | AAGAACTTCTGCGGGTCAGA |
