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. 2001 Jul;159(1):165–178. doi: 10.1016/s0002-9440(10)61683-5

Figure 3.

Figure 3.

Western blotting of the βE11 antigen in primary endothelial cells and tumor-derived cell lines. A: Western blot of ∼500,000 cell equivalents from detergent lysates of bovine retinal endothelial cells growth arrested in a monolayer (lane 1) or at ∼50% confluency (lane 2) probed with anti-βE11 followed by goat anti-mouse IgG-HRP. B: ∼15 μg of tumor cell lysate blotted with anti-βE11 followed by goat anti-mouse IgG-HRP (top). Corresponding Coomassie blue-stained gel is shown in middle panel. Cell lysates are as follows: 1, LNCaP human prostate; 2, Cx.1 human colon carcinoma; 3, murine endothelioma; 4, Lewis lung; 5, murine prostate; 6, rat glioma; 7, B16 BL6 murine melanoma; 8, HEPG2 human liver. Molecular weight markers (in kDa) are indicated to the left of each panel.