Skip to main content
. 2001 Sep;159(3):925–935. doi: 10.1016/S0002-9440(10)61768-3

Figure 6.

Figure 6.

CD40-CD40L interaction induces increased IL-6, IL-8, and MCP-1 production by M5 Thy 1+ fibroblasts. Fibroblasts were serum-starved for 72 hours in fresh media containing 0.5% FBS with or without IFN-γ (500 U/ml). The treatments used for the final 24 hours were: no stimulation, IFN-γ alone (500 U/ml), CD40L alone, or IFN-γ together with CD40L. The anti-CD40L antibody MK13A4 (2.5 μg/ml) was used the final 24 hours on fibroblasts stimulated with IFN-γ and CD40L. Mouse IgG1 was added at 2.5 μg/ml as a control antibody. Supernatants were harvested and tested for IL-6, IL-8, and MCP-1 production by enzyme-linked immunosorbent assay. A: IL-6 production. IFN-γ and CD40L stimulation gave a 3.5-fold increase in IL-6 production over IFN-γ or CD40L stimulation alone. Addition of MK13A4 (anti-CD40L antibody) to cells treated with IFN-γ plus CD40L, completely blocked IL-6 production. B: IL-8 production. Treatment with IFN-γ together with CD40L induced a sevenfold increase in IL-8 production that is blocked by MK13A4. C: MCP-1 production. Addition of IFN-γ with CD40L yielded a fourfold increase in MCP-1 production, which is inhibited by MK13A4. Addition of control antibody did not affect induced IL-6, IL-8, or MCP-1 levels (*, P < 0.05; **, P < 0.01 indicates value is statistically significant).