Figure 5.

RAG1 and RAG2 mRNA expression measured by RT-PCR Southern blot in HT and GD glands. a: Southern blot of RT-PCR products amplified with RAG1 and RAG2 primers and hybridized with the corresponding oligoprobes; bottom, RT-PCR for control GAPDH; only partial normalization was achieved because of the small amount of available sample. Asterisks indicate samples that contained visible GCs. TMB, thymus; CT, esophagus. b: Graph representing the ratio of densitometry values: RAG1, GAPDH (solid bars); RAG2, GAPHDH (open bars), using an inverted image of the GAPDH gel image.