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. 2001 Sep;159(3):1009–1020. doi: 10.1016/S0002-9440(10)61776-2

Figure 4.

Figure 4.

Quantification of myofibroblast markers in granulation tissue by means of Western blot. A: Expression of β-actin, vimentin, and the myofibroblast markers TGFβ-RII, ED-A FN, and α-SMA was investigated. Extracts of 3- to 12-day-old granulation tissue from splinted wounds (sp) are compared to those of unsplinted wounds (un) and of normal dermis (derm). Expression of myofibroblast markers gradually increases in evolving granulation tissues up to 9 days after wounding. No change in expression of β-actin and vimentin is observed. B–D: Protein bands were scanned, quantified by image analysis, and normalized to vimentin. Expression of ED-A FN is significantly higher in 3 day and 6 day splinted tissue compared to the respective control (B), as observed for α-SMA expression at 6 days and 12 days (C). Expression of TGFβ-RII is similar in unsplinted and splinted wound granulation tissue at any age (D). Filled squares indicate P ≤ 0.01 for comparison between unsplinted and splinted tissue of the same age.