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. 2001 Sep;159(3):1171–1180. doi: 10.1016/S0002-9440(10)61793-2

Figure 3.

Figure 3.

Effect of thrombin on IL-8 and MCP-1 secretion by HRPE cell/monocyte co-cultures. A and B: Thrombin enhanced IL-8 (A) and MCP-1 (B) secretion by HRPE cell/monocyte co-cultures. Various concentrations of thrombin (0 to 10 U/ml) or thrombin (10 U/ml) and hirudin (20 U/ml) were added to HRPE cell/monocyte co-cultures (HRPE + Mo). After 24 hours, supernatants were collected and IL-8 and MCP-1 protein levels in supernatants were measured by ELISA. *, P < 0.05; **, P < 0.01, compared with chemokine secretion from HRPE cell/monocyte co-cultures without thrombin (n = 3). HRPE, HRPE cells; Mo, monocytes. C and D: Thrombin-induced IL-8 (C) and MCP-1 (D) secretion was time-dependent. HRPE cells were incubated with (black bars) or without (gray bars) thrombin (10 U/ml) for different times and IL-8 and MCP-1 protein levels in supernatants were measured (n = 3).