Figure 6.

Cell-cell contact enhanced thrombin induction of IL-8 (A), MCP-1 (B), and IL-6 (C) secretion by co-cultures. HRPE cells co-incubated with monocytes in the same cultures, but separated by porous polycarbonate filters (HRPE/Mo), and HRPE cells overlayed with monocytes directly (HRPE + Mo) were incubated with thrombin (10 U/ml). After 24 hours, supernatants were collected and IL-8, MCP-1, and IL-6 protein levels in supernatants were measured by ELISA. a versus c, b versus d, e versus g, f versus h, i versus k, and j versus l; P < 0.01 (n = 3).