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. 2001 Sep;159(3):983–992. doi: 10.1016/s0002-9440(10)61774-9

Figure 2.

Figure 2.

Entry into S phase supported by adhesion to type I collagen. A: Entry into S phase by clones of the X2C2, X2C1, X2C0, X1C1, and control transfectants was measured by BrdU incorporation 24 hours after plating the cells on type I collagen in serum-free MEBM containing recombinant human epidermal growth factor (10 ng/ml), bovine pituitary extract (13 mg/ml), insulin (5 μg/ml), and hydrocortisone (0.5 μg/ml). The percentage of cells that incorporated BrdU during the final 2 hours of incubation was determined. Results shown represent the mean ± SEM for at least three separate experiments. B: Entry into S phase by clones of the X2C0, X2C2, X2C1, and X1C1 transfectants was determined 24 hours after the serum-starved cells were plated on type I collagen in DMEM plus insulin (5 μg/ml). The percentage of cells that incorporated BrdU during the final 2 hours of incubation was determined. Results shown represent the mean ± SEM from at least three separate experiments.