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. 2001 Oct;159(4):1323–1334. doi: 10.1016/S0002-9440(10)62519-9

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Copyright © 2001, American Society for Investigative Pathology

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Figure 9. — Detection of proliferating DPPIV⁺ hepatic cells 6 months after transplantation of ED 14 FLEP cells by dual-label in situ hybridization/immunohistochemistry. Normal rats (A and B) or Rs-treated rats (C and D) were transplanted with 4 × 10 5 ED 14 FLEP cells in conjunction with two-thirds PH. Proliferating transplanted cells were detected by in situ hybridization with a ³⁵S-histone 3 mRNA riboprobe and immunohistochemistry for DPPIV, using a monoclonal antibody, as previously reported. 36 A and C are from liver taken 4 months after cell transplantation and B and D are from liver taken 6 months after cell transplantation. Original magnifications, ×400.