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Western blot analysis of commercial recombinant HGF before (HGF b) and after being added to supernatant of HPMC culture for 12 hours, 24 hours, 36 hours, and 48 hours. The 90-kD band represents monomeric pro-HGF (inactive form); the 70- and 35-kD bands are the two chains of the dimeric active form of HGF; the 66-kD band is BSA.
