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. 2001 Oct;159(4):1275–1285. doi: 10.1016/S0002-9440(10)62514-X

Figure 7.

Figure 7.

Micrograph showing the effect of peritoneal fluid of peritonitic patients on expression of intermediate-type filaments in HPMC. Original magnifications, ×20. Cells were incubated with anti-cytokeratin (A, C, E) or anti-vimentin (B, D, F) antibody, followed by secondary biotinylated antibody and the streptavidin-diaminobenzidine reaction that stains positive cells in brown. Cells cultured with uremic/non-CAPD peritoneal fluid express cytokeratin (A), but not vimentin (B). Addition in the cell cultures of peritoneal fluid of peritonitic patients suppresses the constitutive expression of cytokeratin (C) and causes de novo expression of vimentin (D). Anti-HGF antibody prevents the suppression of cytokeratin (E) and the expression of vimentin (F) induced by peritoneal fluid of peritonitic patients. Note the fibroblastic transformation of cells treated with peritoneal fluid of peritonitic patients.