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. 2000 May 16;97(11):5750–5755. doi: 10.1073/pnas.100107297

Figure 2.

Figure 2

Biochemical properties of Q19-GFP and Q82-GFP. Extracts of animals expressing Q19-GFP and Q82-GFP were separated by native (lanes 1 and 2) and denaturing (lanes 3 and 4) PAGE and immunoblotted with anti-GFP Ab. Q19-GFP exhibits a mobility that corresponds to the size expected for a soluble monomer on native (lane 1) and denaturing (lane 1) gels, whereas Q82-GFP runs as a high molecular mass complex at the top of the native gel (lane 2) and as a monomer on the denaturing gel (lane 4). The arrow indicates a band (lane 2) with the mobility of the Q82-GFP monomer and the asterisk (lane 4) corresponds to GFP alone.