Figure 4.
Soluble TNFR1 inhibits nitric oxide-independent rodent Mφ killing of primed rodent mesangial cells. Murine bone marrow-derived Mφ were co-cultured with mesangial cells as described in Materials and Methods. Experiments were activated with IFN-γ and LPS as described, with L-NIL (30 μmol/L) to block NOS 2-mediated killing, and soluble Fc-fusion proteins TNFR1-Fc, LTβR-Fc, HVEM-Fc, or isotype control IgG1 were added in culture medium at 10 μg/ml. Control wells containing mesangial cells but no Mφ received all reagents. Mesangial cell apoptosis was assessed at 24 hours. Note a reduction in the capacity of Mφ to induce apoptosis in the presence of TNFR1-Fc. *, P < 0.05.