Table 2.
Active TGF-β1 Levels (pM) in Control and TSP2-Null Animals
| TSP2+/+ | TSP2−/− | |
|---|---|---|
| Sponge fluid (day 14) | 1.14 ± 0.21 | 0.85 ± 0.33 |
| Skin (12 weeks) | 1.51 ± 0.34 | 1.15 ± 0.31 |
| Skin fibroblasts | 6.55 ± 3.12 | 4.18 ± 3.46 |
Sponges were implanted subcutaneously in mice. After a 2-week period, the implants were explanted, and the levels of active TGF-β1 in sponge fluid were determined by the PAI-1/luciferase assay. Skin was removed from adult mice and either extracted for determination of active TGF-β1 levels, or processed for isolation of fibroblasts. Conditioned media from fibroblasts grown in culture were harvested and the levels of active TGF-β1 were determined. A total of three sponges, three skin samples, and fibroblast cultures from three individual mice per genotype were analyzed.