Figure 4.

Western blot analysis of SPARC in the culture medium of normal HOSE cells and ovarian cancer cells. Culture medium was collected and concentrated from separate cultures of a primary HOSE cell culture (HOSE713), an immortalized HOSE cell line (HOSE1-15), and four ovarian cancer cell lines (SKOV3, DOV13, OVCA420, and OVCA429). For quantification, culture medium alone (control) and culture medium spiked with different amounts of SPARC (1 μg/ml, 5 μg/ml, 10 μg/ml, and 25 μg/ml) were also included. The estimated concentrations of SPARC in the culture medium of the various cell cultures are indicated at the bottom of the respective lanes. A: Shorter exposure to show the signals obtained from purified human blood platelet SPARC spiked into culture medium. B: Longer exposure to show the signals detected from the culture media of ovarian cancer cells.