Figure 6.

Antiproliferative effects of SPARC on SKOV3 and HOSE cells. Incorporation of BrdU into the DNA of SKOV3 and HOSE1-15 cells after incubation with SPARC for 96 hours was detected by immunoassay. The reaction product, which directly correlates to the amount of DNA synthesis, was quantified by measuring absorbance at 370 nm. Reduction in cellular proliferation of both SKOV3 and HOSE cells after treatment with increasing amounts of SPARC is indicated by the decrease in absorbance at 370 nm (reference wavelength at 490 nm). The values shown are the means ± SEM (bars) of four measurements from one representative experiment. Filled circles, SKOV3 cells; open circles, HOSE1-15 cells.