Figure 8.

Detection of apoptosis in SPARC-treated HOSE cells and SKOV3 cells. A quantitative ELISA that detects mono- and oligo-nucleosomes was used to assay the whole cell lysates prepared from HOSE1-15 cells (A) and SKOV3 cells (B) treated with different amounts of exogenous SPARC. Increase in apoptosis is indicated by the increase in absorbance at 405 nm (reference wavelength 490 nm). SPARC effectively induces apoptosis in SKOV3 cells, but not in HOSE cells. The values shown are the means ± SEM (bars) of duplicate measurements in two independent experiments.