Figure 4.

Immune response in T. cruzi-infected wild-type (open bars) and IL-4−/− (closed bars) mice. A–C: Levels of IFN-γ (A) and IL-2 (B) in culture supernatants, and DNA synthesis (C) in T. cruzi antigen- or Con A-stimulated spleen cell cultures. Spleen cell suspensions were prepared from organs collected at the indicated times after infection. D: Levels of T. cruzi-specific IgM, IgG1, IgG2a, IgG2b, and IgG3 antibodies in sera from individual mice, prepared from blood samples collected seven months after T. cruzi infection. Each bar represents the mean of data obtained from three to six mice. Vertical bars represent standard deviations of means.