Table 2.
Tumorigenicity of Akt and Control Transfectant NIH3T3 Cell Lines
| Clonal cell lines | Soft agar growth* | Tumorigenicity in nude mice† | Latency (days) |
|---|---|---|---|
| Akt-E40K 1 | +++ | 5 /5 | 14–18 |
| Akt-E40K 2 | ++ | 4 /5 | 17–22 |
| Akt-E40K 3 | +++ | 5 /5 | 15–21 |
| Akt-E40K 4 | +++ | 5 /5 | 13–18 |
| Akt-E40K 5 | +++ | 5 /5 | 13–18 |
| Myr-Akt 1 | +++ | 5 /5 | 11–14 |
| Myr-Akt 2 | +++ | 5 /5 | 8–10 |
| Myr-Akt 3 | +++ | 5 /5 | 10–11 |
| Myr-Akt 4 | +++ | 5 /5 | 7–10 |
| Myr-Akt 5 | +++ | 5 /5 | 8–11 |
| Myr-Akt-K179M (1–5) | − | 0 /25 | |
| Vector control (1–5) | − | 1 /25 | 40 |
| WT-Akt (1–5) | − | 2 /25 | 38–42 |
*Number of colony/60 mm plate: 1–10, +; 11–30, ++; >30, +++.
†The number of injected cells: 1 × 106/mouse.