Skip to main content
. 2001 Aug;159(2):449–455. doi: 10.1016/S0002-9440(10)61716-6

Figure 2.

Figure 2.

Northern blot analysis of total RNA prepared from three cases of human colorectal cancer. The mRNAs of LAMP-1 (2455 bp) were analyzed by using the 249-bp probe, mRNA of LAMP-2A (standard form, 1868 bp) by the 166-bp probe, and LAMP-2B (variant form, 4006 bp) by the 154-bp probe. RNA samples were semi-quantitated with ethidium bromide staining of 18S ribosomal bands by using a densitometry computer program. Top: Higher expression of LAMP-1 transcripts in the tumor tissue (T) as compared with its normal counterpart (N) (A and B). Bottom: The density ratios (T/N) of bands derived from tumor (T) and normal (N) samples between 18S rRNA, LAMP-1 (A), and LAMP-2A and -2B (B) (Table 2) ▶ . mRNA from tumors showed greater increase in their density compared with normal counterparts.