Figure 2.

Fumagillin induces apoptosis in mesothelioma cells. A: DNA was extracted from either HUVECs, malignant (HM), or normal (NM-1) mesothelial cells that were exposed for 96 hours to vehicle or fumagillin (0.5 μg/ml). Low-molecular weight DNA was visualized by 2% agarose gel electrophoresis and ethidium bromide staining. MW = 100-bp molecular weight marker. B: Cells were treated with fumagillin (0.5 μg/ml) for 96 hours in complete medium. Nucleosome formation was assessed as indicated in Materials and Methods. One hundred percent corresponded to an optical density reading of 1.278 at 405 nm. Results are mean ± SD from n = 2 done in triplicate.