Figure 5.
Impact of MetAP2 anti-sense oligonucleotide on mesothelioma cell viability and nucleosome formation. A: Mesothelioma cells (HM) were incubated with increasing concentrations of either a specific MetAP2 anti-sense or a scrambled oligonucleotide. After 24 hours total RNA was extracted and blotted with a specific probe for MetAP2. MetAP2 protein levels were determined by Western blotting after 48 hours exposure to the oligonucleotides. β-actin was used as an internal control. B: HM cells were treated for different periods of time with either lipofectin alone (mock) or with 100 nmol/L of the MetAP2 anti-sense or of a scrambled oligonucleotide. At the end of the incubation, cell viability was assessed by trypan blue exclusion. Data points depict mean values ± SD from two experiments with quadruplicate determinations (*, P = 0.02; **, P < 0.001). C: Normal mesothelial (NM-1) and HM cells were treated as indicated in the legend to B. Nucleosome formation in the presence of lipofectin alone, the MetAP2 anti-sense, or the scrambled oligonucleotide was determined after 96 hours using the cell death detection ELISAPLUS kit as indicated in the Materials and Methods section. Results are the average ± SD from three experiments with duplicate determinations. (*, P = 0.018).