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. 2001 Aug;159(2):721–731. doi: 10.1016/S0002-9440(10)61743-9

Figure 7.

Figure 7.

MetAP2 inhibition decreases telomerase activity in mesothelioma cells. A: Mesothelioma (HM) cells were exposed to either 0.5 μg/ml of fumagillin or 100 nmol/L of MetAP2 anti-sense for the indicated periods of time. Telomerase activity in cell extracts (0.1 μg) was determined as described in Experimental Procedures. Values are expressed as percentage of the telomerase activity present in untreated or scrambled oligo-treated HM cells (white bar) and represent the mean ± SD from three experiments with duplicate determinations (*, P = 0.015; **, P < 0.001). B: TRAP assay of mesothelioma (HM) and normal mesothelial (NM-2) cell extracts from cultures treated for 96 hours with either vehicle or 0.5 μg/ml of fumagillin. Negative control refers to HM cell extracts that were incubated with 200 μg/ml of RNase before the TRAP assay. Positive control was 0.02 μg of HeLa cell extract. Results are from one experiment representative of three.