Table 2.
List of Primary Antibodies, Their Sources, Specificities, Dilutions, and Pretreatment Conditions on Methyl Methacrylate-Embedded Semithin Sections as Used in the Present Study
| Antibody (source) | Specificity | Dilution | Incubation, minutes | Pretreatment |
|---|---|---|---|---|
| ED1 (Serotec), monoclonal | Activated macrophages | 1 :100 | 90 | None |
| Iba1, polyclonal 17 | All macrophages | 1 :100 | 180 | None |
| NR4 (Dako), monoclonal | 68-kd neurofilament | 1 :20 | 90 | Microwave 20 minutes, citrate buffer |
| MBP (Serotec), polyclonal | Myelin basic protein | 1 :25 | 90 | Microwave 20 minutes, citrate buffer |
| BrdU (Dako), monoclonal | Proliferating cells | 1 :50 | 120 | Microwave 30 minutes, citrate buffer |
All incubations were at room temperature. Iba1, ionized calcium-binding adaptor molecule 1; MBP, myelin basic protein; BrdU, bromodeoxyuridine.