Figure 1.

Subcellular fractionation of frozen human brain tissue. A: Schematic representation of the subcellular fractionation steps, annotations in B correspond to the fractions outlined in A. B: Parietal cortex (0.4 g) from a human control individual was homogenized and subjected to subcellular fractionation. Twenty μg of each fraction was TCA precipitated (except postnuclear fraction S1 that was loaded directly) and subjected to denaturing 12.5% PAGE. Sequential Western probing was done with 15G7 anti-α-SYN, SY38 anti-synaptophysin (SPH), and 6584 anti-β-SYN, as indicated. Data are representative for three different control cortex fractionations.