Table 2.

α-SYN Expression Levels in Transgenic Mice

Transgenic construct	Line	α-SYN transgene copy number	Transgenic α-SYN mRNA [% actin]	Transgenic α-SYN protein [ng/μg]	Endogenous α-SYN protein [ng/μg]
(Thy1)-h[wt]α-SYN	6	7	175	1.93 ± 0.31	1.35 ± 0.10
(Thy1)-h[wt]α-SYN	10	85	65	0.80 ± 0.31	1.61 ± 0.14
(Thy1)-h[wt]α-SYN	14	13	190	2.02 ± 0.11	0.65 ± 0.06
(Thy1)-h[wt]α-SYN	23	2	130	1.32 ± 0.17	0.43 ± 0.21
(Thy1)-h[wt]α-SYN	38	5	130	1.58 ± 0.60	0.81 ± 0.28
Nontransgenic		N/A†	N/A	N/A	1.77 ± 0.55
(Thy1)-h[A30P]α-SYN	8	15–25	370	1.20 ± 0.60	1.42 ± 0.99
(Thy1)-h[A30P]α-SYN	9	5	30	0.39 ± 0.05	1.64 ± 0.05
(Thy1)-h[A30P]α-SYN	14	10	105	1.2*	n.d.
(Thy1)-h[A30P]α-SYN	18	10–15	170	1.43 ± 0.18	1.59 ± 0.00
(Thy1)-h[A30P]α-SYN	31	15–20	155	1.69 ± 0.31	1.26 ± 0.01

Five lines each of [wt]α-SYN and [A30P]α-SYN-expressing transgenic mice stably transmitted the transgene. The integrated transgene copy number was determined by quantitative Southern blotting, transgenic α-SYN mRNA expression levels relative to β-actin by quantitative Northern blotting, and the amount of transgenic and endogenous α-SYN protein by quantitative Western blotting. α-SYN protein was measured in three different mouse whole-brain cytosol samples on the same blot. Results are expressed as mean ± SD.

*Determined indirectly; expression levels relative to the other lines.

^†N/A: not applicable.