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. 2001 Dec;159(6):2001–2009. doi: 10.1016/S0002-9440(10)63051-9

Figure 3.

Figure 3.

Decreased expression of TJ and AJ proteins in IBD colonic mucosa is restricted to epithelium with actively transmigrating PMN. Immunohistochemistry was used to map the distribution of TJ and AJ proteins, JAM (a, b, c), claudin-1 (d, e, f), E-cadherin (g, h, i), and β-catenin (j, k, l) in normal and actively inflammed IBD colonic mucosa. JAM and claudin-1 in normal mucosa are expressed in focal concentrations at the apical region of the lateral membranes of epithelial cells consistent with its localization in TJs. Additional pool of these proteins are identified in the lateral membrane subjacent to the TJ in the AJ. The AJ proteins, β-catenin and E-cadherin, are abundantly expressed in lateral membranes of normal epithelial cells. In active UC, expression of all four proteins was diminished exclusively in epithelial cells adjacent to actively transmigrating PMN (arrowheads). However, no change in expression or distribution of these TJ and AJ proteins was observed in epithelial cells not exposed to transmigrating PMN. Arrows, TJ, AJ; arrowhead, PMN. Scale bar, 15 μm